Fig. 9
From: 3D-environment and muscle contraction regulate the heterogeneity of myonuclei
Schematic of molecular and cellular heterogeneity of myonuclei in a dish. In foetal limb muscles, COL22A1, FGF4, NES, ANKRD1 and MEF2C transcripts are located in tip myonuclei. In a dish, the expression of COL22A1 and FGF4 is lost, while the expression of NES, ANKRD1 and MEF2C is maintained in tips of cultured foetal myotubes. In limb muscles, fibroblast incorporation is observed preferentially at muscle tips, while there is no regionalisation of fibroblast incorporation in myotubes in fibroblast/myoblast co-cultures. Myotubes are in pink and tendons in green. Tip gene expression is schematized in purple. Myoblast nuclei and myonuclei are in red and fibroblast nuclei are in green