Fig. 8
From: 3D-environment and muscle contraction regulate the heterogeneity of myonuclei
Inhibition of muscle contraction modified the expression of genes expressed in tip myonuclei of foetal muscles. Chicken embryos were treated with DMB at E7.5 to block muscle contraction and analysed 48 h after treatment at E9.5 (N = 3). A-D In situ hybridization to transverse limbs sections at the level of the zeugopod of control (A,C) and DMB-treated (B,D) E9.5 chicken embryos, with COL22A1 (A,B) and FGF4 (C,D) probes (blue) followed by immunostaining with the MF20 antibody to label myosins (light brown). The expression of COL22A1 and FGF4 was lost in paralyzed muscles (B,D) compared to control muscles (A,C). Note that COL22A1 expression is maintained in cartilage and perichondrium in paralyzed limbs (B) as in control limbs (A). E, E’, F,F’ Fluorescent situ hybridization to transverse limbs sections at the level of the zeugopod of control (E,E’) or DMB-treated (F,F’) E9.5 chicken embryos, with NES probe (red) followed by immunostaining with the MF20 antibody to label myosins (green) and stained with DAPI (blue). (E’,F’) are high magnification of the FCU muscles of E (control) and F (DMB-treated) limbs. u, ulna, r, radius