Fig. 7
From: 3D-environment and muscle contraction regulate the heterogeneity of myonuclei
The regionalized expression of NES, ANKRD1 and MEF2C in cultured myotubes is not changed in the presence of fibroblasts. A-C Fluorescent in situ hybridization to quail myoblast / chicken fibroblast co-cultures with the NES (A), ANKRD1 (B) and MEF2C (C) probes (red), followed by an immunohistochemistry with the MF20 antibody to label myosins (green) combined with DAPI staining (blue). D,D’ High magnification of the area squared in A, showing in the top panel, (NES transcripts, red, myosins, green) and DAPI staining, blue) combined with immunolabelling of quail nuclei (grey) (D), and in the bottom panel, immunolabelling of quail nuclei (grey) combined with DAPI staining, (blue) (D’). Arrowheads point to chicken fibroblast myonuclei expressing NES, while arrows point to point to chicken fibroblast myonuclei not expressing NES. E Fluorescent in situ hybridization to quail myoblast / chicken fibroblast co-cultures with NES probes (red) followed by an immunolabelling of myosins (green) and of quail nuclei (grey), combined with DAPI staining (nuclei, blue). F,F’,F” High magnification of the area squared in E, showing NES transcripts, (red), myosins, (green) and DAPI staining (blue) combined with immunolabelling of quail nuclei (grey) (F), showing myosins, (green) and DAPI staining (blue) combined with immunolabelling of quail nuclei (grey) (F’) and showing NES transcripts, (red), DAPI staining (blue) combined with immunolabelling of quail nuclei (grey) (F”). (F,F’,F”) Arrows points to a chicken fibroblast myonuclei with low/residual levels of NES incorporated into a quail myotube