Fig. 6
From: 3D-environment and muscle contraction regulate the heterogeneity of myonuclei
The regionalized expression of NES, ANKRD1 and MEF2C in tip myonuclei of limb muscles is maintained in cultured myotubes. A-D Gene expression for muscle tip genes (NES, ANKRD1, MEF2C) and tendon gene (SCX) in transverse muscle sections from E10 chicken limbs with fluorescent in situ hybridization for NES probe (A) and with colorimetric in situ hybridization for ANKRD1 (B), MEF2C (C) and SCX (D) probes (dark grey), followed by an immunohistochemistry with the MF20 antibody to label myosins (green). E Fluorescent in situ hybridization to quail myoblast cultures with NES probe (red) followed by an immunolabelling of myosins (green) combined with DAPI staining (nuclei, blue). F-G Fluorescent in situ hybridization to chicken myoblast cultures with ANKRD1 (F), MEF2C (G), MYOG (H) probes (red) followed by an immunolabelling of myosins (green) combined with DAPI staining (nuclei, blue). E’-H’ are high magnifications of squared areas in (E–H). Arrows point to NES (E’), ANKRD1 (F’) and MEF2C (G’) expression at myotube tips in low myosins-expressing zones, while MYOG transcripts (F’) are expressed in myosins-positive zones