Fig. 3
From: 3D-environment and muscle contraction regulate the heterogeneity of myonuclei
Recruitment of fibroblast nuclei into myotubes in fibroblast/myoblast co-cultures. (A,A’,A”) Quail myoblast and chicken fibroblast and co-cultures immunolabelled to quail nuclei (QCPN antibody, red) and myosins (MF20 antibody, green), combined with DAPI staining (nuclei, blue). A Left panel shows quail nuclei/Myosins/DAPI, while right panels (A’,A”) are high magnification squared in A, showing quail nuclei/Myosins/DAPI staining (A’) and quail nuclei/Myosins (A”). Arrows point to chicken fibroblast myonuclei. B Percentage of chicken fibroblast myonuclei versus quail and chicken myonuclei and versus total chicken nuclei. Graph shows mean ± s.d. (C,C’,C”) Chicken myoblast and quail fibroblast and co-cultures immunolabelled to quail nuclei (QCPN antibody, red) and myosins (MF20 antibody, green), combined with DAPI staining (nuclei, blue). C Left panel shows quail nuclei/Myosins/DAPI, while right panels (C’,C”) are high magnification squared in C, showing quail nuclei/Myosins/DAPI staining (C’) and Myosins/DAPI (C”). Arrows point to quail fibroblast myonuclei. D Percentage of quail fibroblast myonuclei versus chicken and quail myonuclei and versus total quail nuclei. Graph shows mean ± s.d. (E,E’,E”,F,F’,F”) Fluorescent in situ hybridization to quail myoblast / chicken fibroblast co-cultures with MYOG (E,E’,E”) or TMEM8C (F,F’,F”) probes (red) followed by an immunolabelling of myosins (green) and of quail cells (grey) combined with DAPI staining (nuclei, blue). Arrows point to chicken fibroblast myonuclei expressing MYOG (E,E’,E”) and TMEM8C (F,F’,F”)