Fig. 2
From: 3D-environment and muscle contraction regulate the heterogeneity of myonuclei
Fibroblasts promote myoblast differentiation in fibroblast/myoblast co-cultures. A Percentage of quail myoblasts and chicken fibroblasts in co-cultures after five days of cultures. Representative field of quail nuclei (QCPN antibody, red) and chicken nuclei (DAPI, blue). B Quail myoblast / chicken fibroblast co-cultures immunolabelled to PAX7 (PAX7 antibody, red) and myosins (QCPN antibody, green) combined with DAPI staining (blue). C Quail myoblast / chicken fibroblast co-cultures immunolabelled to MYOG (red) and myosins (QCPN antibody, green) combined with DAPI staining (blue). D,E Percentage of PAX7-positive nuclei (D) and of MYOG-positive nuclei (E) versus total nuclei in fibroblast/myoblast co-cultures. F Fusion index of quail cells in co-cultures. Graphs show mean ± s.d. G,H Myoblast cultures (G) and myoblast/fibroblast co-cultures (H) labelled with the MF20 antibody (myosins, green), combined with DAPI staining (nuclei, blue). I Quantification of myotube area per surface unit in myoblast cultures and myoblast/fibroblast co-cultures. Graph shows mean ± s.d. J,K Fluorescent in situ hybridization to quail myoblast cultures (J) and to quail myoblast / chicken fibroblast co-cultures (K) with TMEM8C probe (red) followed by an immunolabelling of myosins (green) combined with DAPI staining (nuclei, blue)