Fig. 1
From: 3D-environment and muscle contraction regulate the heterogeneity of myonuclei
Loss of regionalisation of fusion-associated genes in cultured myotubes. A-D Fluorescent in situ hybridization to adjacent transverse muscle sections from E10 chicken embryos with TMEM8C (A), MYOG (B), MYOD (C) and SCX (D) probes (red), followed by an immunolabelling of myosins (green) (D), combined with DAPI staining (nuclei, blue). White dashed lines correspond to the muscle/tendon interface. E-J Primary cultures of limb myoblasts from E10 quail embryos. E,F Myoblasts and myotubes labelled with PAX7 (muscle progenitors, red) and MF20 (myosins, green) antibodies (E), or with MYOG (red) and MF20 (myosins, green) antibodies (F), combined with DAPI staining (nuclei, blue). G Percentage of PAX7-positive nuclei versus total nuclei. Percentage of MYOG-positive nuclei versus total nuclei. Fusion index. Graphs show mean ± s.d. H-J Fluorescent in situ hybridization to quail myoblast cultures with TMEM8C (H), MYOG (I) and MYOD (J) probes (red) followed by an immunolabelling of myosins (green) combined with DAPI staining (nuclei, blue). K-M Fluorescent in situ hybridization to chicken myoblast cultures with TMEM8C (K), MYOG (L) and MYOD (M) probes (red) followed by an immunolabelling of myosins (green) combined with DAPI staining (nuclei, blue). (K’-M’) are high magnification of squared regions in (K-M)