Fig. 8

GsMTx4-blocked PIEZO1 channel inhibits calcium overload in myofibers of mdx mice. (A) Representative immunohistochemical images showed that Piezo1 was expressed in the sarcolemma of mdx mice. (B-C) Representative Western blot of CaMKII and p-CaMKII after treatment with GsMTx4 and Yoda1 in mdx mice, and the relative p-CaMKII/CaMKII level was significantly increased decreased after treatment with GsMTx4 and increased after treatment with Yoda1 in mdx mice (n = 5; mean ± SD; Student’s t-test). (D) Representative immunohistochemical images after treatment with GsMTx4. (E) The relative calcium influx in isolated single myofiber was significantly restrained after treatment with GsMTx4 (n = 5; mean ± SD; One-way ANOVA). (F) Representative immunohistochemical images after treatment with Yoda1. (G) The relative calcium influx in isolated single myofiber was significantly increased after Yoda1 was added (n = 5; mean ± SD; One-way ANOVA). Statistical significance was set at P < 0.05. *P < 0.05. Scale bar, 50 μm