Fig. 7

GsMTx4-blocked PIEZO1 channel promotes myogenic differentiation through YAP-MyoG axis. (A) Representative immunofluorescence images of YAP after myogenic differentiation after treatment with GsMTx4 for 3 days. (B) The YAP N/C ratio was increased after treatment with GsMTx4 (n = 114 ∼ 145; mean; Student’s t-test). (C) Representative immunofluorescence images of YAP after myogenic differentiation after treatment with Yoda1 for 3 days. (D) The YAP N/C ratio was decreased after treatment with Yoda1, and XMU-MP-1 partially rescued the suppressive effect of Yoda1 on YAP N/C ratio (n = 85 ∼ 109; mean; One-way ANOVA). (E) XMU-MP-1 treatment partially rescued the suppressive effect of Yoda1 on Myh4 and Myh7 expression (n = 5; mean ± SD; Student’s t-test). (F) XMU-MP-1 treatment partially rescued the suppressive effect of Yoda1 on MyoG expression (n = 5; mean ± SD; Student’s t-test). (G) Immunofluorescence colocalization analysis showed that YAP and MyoG were aggregated in the nuclei of the differentiated myotubes. (H) Correlation analysis showed that the YAP N/C ratio was positively correlated with the mean intensity of the MyoG nucleus (n = 107; Pearson correlation coefficient method). (I) Mymk and Mymx expression were increased after treatment with GsMTx4 (n = 5; mean ± SD; Student’s t-test). (J) Mymk and Mymx expression were decreased after treatment with Yoda1 (n = 5; mean ± SD; Student’s t-test). Statistical significance was set at P < 0.05. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ^###P < 0.001. Scale bar, 50 μm