Fig. 6

GsMTx4-blocked PIEZO1 channel promotes myogenic differentiation in vivo. (A) Flow diagram of the experiment in WT mice, the red markings indicate the time points for drug injections. (B) Representative H&E images show myofibers treated with GsMTx4 and Yoda1. (C) The weight of tibialis anterior muscle was increased after treatment with GsMTx4 and decreased after treatment with Yoda1 (n = 5; mean ± SD; Student’s t-test). (D) The cross-sectional area of myofibers was increased after treatment with GsMTx4 and decreased after treatment with Yoda1 (n = 5; mean ± SD; Student’s t-test). (E) The proportion of centrally nucleated fibers was decreased after treatment with GsMTx4 (n = 5; mean ± SD; Student’s t-test). (F) Piezo1 expression was increased after treatment with Yoda1 (n = 5; mean ± SD; Student’s t-test). (G) Pax7 expression was decreased after treatment with GsMTx4 and increased after treatment with Yoda1 (n = 5; mean ± SD; Student’s t-test). (H) The proportion of PAX⁺ cells was increased after treatment with Yoda1 (n = 5; mean ± SD; Student’s t-test). Statistical significance was set at P < 0.05. *P < 0.05; **P < 0.01. Scale bar, 50 μm