Fig. 5

Activating PIEZO1 channel inhibits myogenic differentiation in vitro. (A) Representative immunofluorescence images of Ki67 for GsMTx4 treatment satellite cells. (B) GsMTx4 treatment decreased the percentage of Ki67-positive cells, and Yoda1 treatment increased the percentage of Ki67-positive cells (n = 5; mean ± SD; Student’s t-test). (C) Cell counts analysis showed consistent trends with the immunofluorescence analysis of Ki67 (n = 5; mean ± SD; Student’s t-test). (D) qPCR analysis showed that Piezo1 was upregulated in plated satellite cells at Day 2 and declined gradually at Day 5 (n = 5; mean ± SD; One-way ANOVA). (E) Representative immunofluorescence images showed that Yoda1 treatment restrained myogenic differentiation in a concentration-dependent manner. (F) The MyoG positive nuclei index was decreased after treatment with Yoda1 (n = 5; mean ± SD; One-way ANOVA). (G) The myotube width were decreased after treatment with Yoda1 (n = 5; mean ± SD; One-way ANOVA). (H) qPCR showed that the expression levels of Myh1, Myh2, Myh4 and Myh7 were decreased after treatment with Yoda1 (n = 5; mean ± SD; One-way ANOVA). Statistical significance was set at P < 0.05. *P < 0.05; **P < 0.01; ***P < 0.001. Scale bar, 50 μm