Fig. 4

GsMTx4-blocked PIEZO1 channel ameliorates muscular dystrophy in mdx mice. (A) Representative Western blot of dystrophin in muscle tissue of WT and mdx mice. (B) Flow diagram of the treatment in mdx mice, the red markings indicate the time points for drug injections. (C) Representative H&E images show myofibers treated with GsMTx4 for 30 days. (D-F) The cross-sectional area of myofibers and grip strength were increased, and the proportion of centrally nucleated fibers was decreased after treatment with GsMTx4 (n = 5; mean ± SD; Student’s t-test). (G) Representative H&E images show myofibers treated with Yoda1 for 30 days. (H-J) The cross-sectional area of myofibers and grip strength were decreased after treatment with Yoda1 (n = 5; mean ± SD; Student’s t-test). Statistical significance was set at P < 0.05. *P < 0.05. Scale bar, 50 μm