Fig. 2

PIEZO1 is preferentially expressed in activated satellite cells. (A) The expression pattern of Piezo1 in different subsets of cells in skeletal muscle according to single-nucleus RNA sequencing dataset showed that Piezo1 was predominantly highly expressed in satellite cells, endothelial cells, and tenocytes. (B) Immunofluorescence colocalization analysis showed that Piezo1 was preferentially expressed on the cell membrane of satellite cells in vivo. (C) Immunofluorescence colocalization analysis showed that Piezo1 was preferentially expressed on the cell membrane of satellite cells in plated satellite cells. (D) Immunofluorescence colocalization analysis showed that Piezo1 was preferentially expressed on the cell membrane of satellite cells of single myofiber. (E) qPCR showed that the mRNA expression of Piezo1, Pax7, and MyoD were upregulated in muscle tissue after CTX injection (n = 5; mean ± SD; Student’s t-test). Statistical significance was set at **P < 0.01. Scale bar, 50 μm