Fig. 1

Upregulated PIEZO1 in skeletal muscle of DMD patients. (A) The expression of mechanosensitive calcium channels, including members of the transient receptor potential channel family and Piezo channel family (n = 5; mean; Student’s t-test). (B) The expression of PIEZO1 was upregulated in muscle tissue of DMD patients according to GEO datasets GSE6011 (n = 14 ∼ 23; mean ± SD; Student’s t-test). (C) The expression of PIEZO1 was upregulated in muscle tissue of DMD patients according to GEO datasets GSE38417 (n = 6 ∼ 16; mean ± SD; Student’s t-test). (D) The expression of PIEZO1 was upregulated in the muscle tissue of DMD patients according to qPCR (n = 6; mean ± SD; Student’s t-test). (E-F) Representative Western blot of PIEZO1 in muscle tissue of DMD patients, and the relative PIEZO1 level was significantly increased in DMD patients (n = 3; mean ± SD; Student’s t-test). Statistical significance was set at P < 0.05. *P < 0.05; **P < 0.01; ****P < 0.0001