Fig. 2

Assessment of muscle regeneration and MuSC senescence in injured and control muscles upon FSK treatments. A Immunofluorescence for Laminin (red) and eMHC (green) on TA harvested at 14 DPI from WT, DMD, DMD treated with FSK short term (FSK S-T) or long term (FSK L–T). Scale bar 20 µm. B Quantification of the number of eMHC + fibers per mm². C Immunofluorescence for KI67 (red), PAX7 (green) and DAPI on TA harvested at 14 DPI from WT, DMD, DMD treated with FSK short term (FSK S-T) or long term (FSK L–T). Scale bar 25 µm. D Quantification of the number of PAX7 + cells per mm². E Quantification of the percentage of PAX7 + : KI67 + cells. F) Quantification of the percentage of PAX7 + : γH2AX + cells