Fig. 7

IBA-1 positive cell area in PK007 treated GA and EDL mdx muscles is significantly decreased. Representative images of IBA-1-labelled macrophages (red) with DAPI-labelled nuclei (blue) in PK007 and vehicle-treated mdx mice for the 3 hindlimb muscles; GA (A, B), TA (C, D) and TA (E, F). Scale bars 10 µm. Quantification (G) shows the average IBA-1-positive cell area per muscle (proportion of total muscle area) (mean ± SEM). Panel (H) represents the quantification of IBA-1 + /iNOS + macrophage area (M1 macrophage area) in the GA, TA, and EDL muscles of PK007- and vehicle-treated mdx mice. A Student’s t-test was performed between PK007 treated (green bars; GA: n = 10, TA: n = 10, EDL: n = 10) and vehicle-treated (orange bars; GA: n = 8, p = 0.0475; TA: n = 9, p = 0.0908; EDL: n = 9, p = 0.0342) to determine significance. Images were captured at 20 × objective on the Leica DMi8 SP8 inverted widefield microscope and magnified for quantification