Fig. 6

No significant change in fibrosis in wild-type and mdx muscles with Picrosirius Red staining. Representative transverse sections of hindlimb muscles stained with Picrosirius Red, showing collagen deposition in (A) wild-type (WT, purple), (B) vehicle-treated mdx (orange), and (C) PK007-treated mdx mice (green). Red fibres indicate mature type I collagen, while the black arrow highlights an area of collagen deposition, i.e., fibrosis. Insets display the full muscle cross-sections. Quantification of fibrosis as a percentage of total muscle area shows no significant differences in fibrosis percentages in the GA (D), TA (E), and EDL (F) muscles across PK007-treated, Vehicle-treated mdx, and WT mice. Data are presented as mean ± SEM, with individual points representing biological replicates (n = 4). Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test (p > 0.05)