Fig. 5
From: Generation of a novel mouse model of nemaline myopathy due to recurrent NEB exon 55 deletion
Hmz-NebΔExon55 TA fiber type composition shift. Comparing skeletal muscle fiber composition in (n = 4) ~ 2-month-old Hmz-NebΔExon55 male mice TA muscle. Isopentane flash frozen horizontal TA sections were stained for myosin heavy chain (MHC) type IIb, IIa, and I protein. n = 8 representative 20X magnification immunofluorescence images were taken per sample and quantified by hand in ImageJ for fiber content. HOM animals see a significant decrease (P = 0.029) in type IIb fast fiber composition and a significant increase in type IIa (P = 0.0429) and I (P < 0.001) slow fiber composition with no significant (P = 0.6114) change in type IIx composition