Fig. 4
From: Generation of a novel mouse model of nemaline myopathy due to recurrent NEB exon 55 deletion
Hmz-NebΔExon55 intact muscle mechanics analysis. Intact muscle mechanics on male and female WT, HET, and HOM Hmz-NebΔExon55 EDL and SOL tissue. (A) Force frequency measurements on EDL and SOL muscle. Muscles were stretched to optimal length (L0) and then stimulated at incremental stimulation frequencies. Forces measure in mN and normalized to physiological cross-sectional area (PCSA) indicate that there is a large magnitude reduction in HOM EDL maximal force generation potential and a moderate reduction in HOM SOL maximal force generation potential that is more pronounced in male animals. (B) Muscle fatigue measurements on EDL and SOL muscle. Muscles were stretched to optimal length (L0) and then stimulated at constant 150 Hz (SOL) and 200 Hz (EDL) tetani every 3 s for 74 repetitions. Fatigue measurements indicate that there is a significant increase in fatigue resistance in EDL muscle (P = 0.0089) and a trend towards increased fatigue resistance in SOL muscle (P = 0.0832) compared to WT controls