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Fig. 1 | Skeletal Muscle

Fig. 1

From: Endurance exercise with reduced muscle glycogen content influences substrate utilization and attenuates acute mTORC1- and autophagic signaling in human type I and type II muscle fibers

Fig. 1

Workflow of the one- and two-legged exercise and muscle fiber isolation, identification, and analysis. A. Subject characteristics and schematic description of the exercise protocol, B. Individual fibers were separated from freeze-dried muscle biopsies, classified by dot-blotting and pooled into groups of type I and type II fibers. The fiber pools were weighed and homogenized in western blot buffer (~ 1 µg µl− 1) and analyzed with regard to proteins in the mTORC1 pathway as well as substrate and metabolites using metabolomics, C. Number and proportion of type I and type II fibers dissected out from biopsies obtained from the low and normal leg pre- and post-exercise, D. The purity of type I and type II fiber pools confirmed by analyzing the homogenates with antibodies against MyHC I or MyHC II, E. The one-legged exercise resulted in a 60–65% reduction in muscle glycogen the following morning, #P < 0.001 for low versus normal leg, and F. Fiber-type specific differences in protein and metabolite levels in biopsies from the normal leg pre-exercise, *P < 0.05 for type II versus type I fibers

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Skeletal Muscle

ISSN: 2044-5040

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