Fig. 2

Sgcd^−/− mice exhibit early (1 month) regeneration followed by later (2 months) fiber size reduction. A Fiber size distribution measured by minimum Feret’s diameter in laminin-stained muscle sections. Values represent the percentage of fibers (mean ± SEM) in a given diameter class (5 μm/class). Mice were compared at different ages using two-way ANOVA with Tukey’s multiple comparisons post-test (n = 3; Supplementary Table 1). B Measurement of Myh3 gene expression and eMHC protein levels. Data are normalized to gapdh (n = 3–5) and GAPDH (n = 3), respectively. A two-way ANOVA with Tukey’s multiple comparison post-test and an unpaired two-tailed t-test were used to compare genotypes of mice at each age and muscle for gene and protein expression, respectively. Values represent mean ± SEM; *p ≤ 0.05, **p ≤ 0.01, and ***p ≤ 0.001. C Representative immunofluorescence images of centrally nucleated TR fibers stained with laminin (fibers, red) and Hoechst (nuclei, blue). Scale bar: 100 μm. D Representative immunofluorescence images of regenerated TR fibers stained with embryonic myosin heavy chain (eMHC, red) and laminin (green). one-month Scale bar: 1000 μm; two-month Scale bar: 1250 μm