Fig. 2

Effects of short-term NMES training on MuSCs and myonuclear content. A Immunostaining for laminin (red), Pax7 (green), EdU (white) and Hoechst (blue) on gastrocnemius muscle section from mice submitted either to the control procedure or to NMES. Arrowheads show Pax7^posEdU^pos cells. B Number of Pax7^pos cells per myofiber in control (n = 13) and NMES (n = 13) mice. C Number of Pax7^posEdU^pos cells per myofiber in control (n = 5) and NMES (n = 4) mice. D Immunostaining for laminin (red), PCM1 (green), EdU (white) and Hoechst (blue) on gastrocnemius muscle section from mice submitted either to the control procedure or to NMES. Arrowheads show PCM1^posEdU^pos cells. E Number of myonuclei (i.e., PCM1^pos cells below the basal lamina) per myofiber in control (n = 13) and NMES (n = 12) mice. F Number of newly fused myonuclei (i.e., PCM1^posEdU^pos cells located below the basal lamina) per myofiber. G Proportion of newly fused myonuclei (% of total PCM1^pos cells) in control (n = 5) and NMES (n = 4) mice. Scale bar = 25 µm. Significantly different from control:^* P < 0.05; ^*** P < 0.001. Values are reported as mean ± SD