Fig. 3
From: Wnt7a is required for regeneration of dystrophic skeletal muscle
Wnt7a-EVs exhibit enhanced bioactivity. (A) Schematic showing the experimental protocol for ex vivo EV treatment of single myofibers isolated from FDB muscles, imaging and analysis using high-content screening. (B) Representative immunofluorescence images of symmetric (top panel) and asymmetric (bottom panel) satellite stem cell divisions. (C) Rate of muscle stem cell division after 42 h in culture is not altered by treatment with either Wnt7a-EVs or with recombinant Wnt7a. (D) Symmetric satellite stem cell divisions are significantly increased by Wnt7a-EV treatment. EVs (extracellular vesicles), FDB (Flexor Digitorum fibers). n\(\:\ge\:\)5 replicates, mean ± s.e.m., p values determined by two-sided Student’s test (*p<0.05, **p < 0.01, ***p < 0.001, n.s.=not significant)