Fig. 1

Lack of Wnt7a expression impairs muscle regeneration. (A-left) H&E stained sections of injured and non-injured TA muscle of mdx mice at 3 dpi. (A-right) Wnt7a immunostained (green) sections of injured and non-injured TA from mdx mice upon 3 dpi. Laminin (red) delineates myofibers. Nuclei are stained with DAPI. (B) Mouse strains used and experimental design. (C and D) Quantification of Pax7-expressing cells in regenerating WT muscle. Scale bar 50 μm. (E and F) Quantification of Pax7-expressing cells in regenerating mdx TA muscle. Scale bar 50 μm. (G and H) Myofiber caliber distribution at 21 d following regeneration in WT and mdx TA muscle with and without Wnt7a deletion. (I and J) H&E stained sections at 21 d following regeneration in WT and mdx TA muscle with and without Wnt7a deletion. Scale bar 100 μm. TA (Tibialis Anterior), dpi (days post injury). n ≥ 4 mice, mean ± s.e.m., p values determined by two-sided Student’s t-test (*p<0.05,**p < 0.01, n.s.=not significant)