Fig. 8

Muscle-specific deletion of GLUT4 impairs myofiber size at 14 days post BaCl₂-induced injury. In 13–14 week old, male control (CON) and muscle-specific GLUT4 knockout (mG4KO) mice, BaCl₂ solution was injected into the muscles of one leg. The contralateral leg was not injected. (A + B) Representative images of muscle cross-sections with the myofiber cell perimeter immunolabeled for laminin (violet) and the nuclei stained with DAPI (green) for the (A) 7 day post-BaCl₂ injured muscles, and for the (B) 14 day post-BaCl₂ injured muscles. Scale bar = 200 μm. (Panels C-J) Quantification of the average myofiber cross-sectional areas (CSA) and percent frequency of myofibers with CSAs divided into groups of 450–500 um². (C + D & G + H) Data from mice euthanized 7 days after BaCl₂ injections (N = 4–6 muscles/group). (E + F & I + J) Data from mice euthanized 14 days after BaCl₂ injections (N = 6–7 muscles/group). Data are presented as individual data points plus the mean ± standard deviation, or as mean ± standard deviation. Statistical significance was defined as P < 0.05, determined by t-tests, and denoted by ‘*’ vs. non-injected control muscle (-)