Fig. 7

Muscle-specific deletion of GLUT4 does not alter muscle fibrosis post BaCl₂-induced injury. In 13–14 week old, male control (CON) and muscle-specific GLUT4 knockout (mG4KO) mice, BaCl₂ solution was injected into the muscles of one leg. The contralateral leg was not injected. (Panels A + B) Representative images of tibialis anterior muscle cross-sections immunolabeled for type 1 collagen (red) and stained with DAPI to label nuclei (green) for the (A) 7 day post-BaCl₂ injured muscles and for the (B) 14 day post-BaCl₂ injured muscles. Scale bar = 200 μm. (Panels C + D) Quantification of the percent area of type 1 collagen in the muscles from the (C) 7 day post-BaCl₂ injected mice (N = 4–5 muscles/group), and (D) 14 day post-BaCl₂ injected mice (N = 4–6 muscles/group). Data are presented as individual data points plus the mean ± standard deviation. Statistical significance was defined as P < 0.05, determined by Two-Way ANOVA with Tukey’s posthoc analysis, and denoted by ‘*’ vs. non-injected control muscle (-)