Fig. 5

Muscle-specific deletion of GLUT4 impairs muscle glucose uptake at 14 days post BaCl₂-induced injury. In 13–14 week old, male control (CON) and muscle-specific GLUT4 knockout (mG4KO) mice, BaCl₂ solution was injected into the muscles of one leg. The contralateral leg was not injected. Muscles were examined at 7 days or 14 days post-injury. (Panels A + B) Extensor digitorum longus muscles were incubated in [³H]-2-deoxyglucose to assess glucose uptake. (Panels C + D) Tibialis anterior muscles were frozen to assess GLUT4 protein levels by immunoblot (IB) analyses. Representative images and quantification of band intensity. Data are presented as individual data points plus the mean ± standard deviation, with N = 7–9 muscles/group. Statistical significance was defined as P < 0.05, determined by Two-Way ANOVA with Tukey’s posthoc analysis, and denoted by ‘*’ vs. non-injected control muscle (-), and ‘#’ vs. CON mice