Fig. 4

Body weight and body composition are impaired in muscle-specific GLUT4 knockout mice. All measures were made in 11–12 week old, male control (CON) and muscle-specific GLUT4 knockout (mG4KO) mice. (A) Tissues were collected to assess GLUT4 protein levels by immunoblot (IB) analysis. Images from N = 3 mice/group. (B) Body weight. (Panels C-F) Body composition was assessed by EchoMRI™. (C) Lean mass. (D) Fat mass. (E) Percent lean mass relative to body weight. (F) Percent fat mass relative to body weight. (G) Tibias were isolated and tibia length measured with a micrometer. Data are presented as individual data points plus the mean ± standard deviation, with N = 10–13 mice/group. Statistical significance was defined as P < 0.05, determined by t-tests, and denoted by ‘*’ vs. CON mice