Fig. 3
From: Regulation of injury-induced skeletal myofiber regeneration by glucose transporter 4 (GLUT4)
BaCl2-induced muscle injury stimulates a time-dependent alteration in muscle GLUT4 protein levels. In male, wild-type C57BL/6J mice, 50 µl of a 1.2% BaCl2 solution was injected into the tibialis anterior (TA) muscles of one leg. The contralateral leg was not injected. After 3, 5, 7, 10, 14 or 21 days, TA muscles were collected and protein extracted to assess total muscle GLUT4 protein levels by immunoblot (IB) analyses. Data are presented as individual data points plus the mean ± standard deviation, with N = 6 muscles/group. Statistical significance was defined as P < 0.05, determined by Two-Way ANOVA with Tukey’s posthoc analysis, and denoted by ‘*’ vs. non-injected control muscle (-), ‘a’ vs. 3 days, and ‘b’ vs. 5 days