Fig. 3

Exogenous administration of recombinant myonectin protein aggravates acinar cell necrosis. (A) PACs were treated with CCK, together with gradient doses of Myonectin for 6 h; the levels of LDH release are shown. (B) Immunofluorescence imaging of pancreatic acinar cells stained with Calcein-AM/PI (1200x). (C) Representative images of pancreatic tissue H&E staining in PDL mice after treatment with gradient doses of recombinant Myonectin protein. (D) Pathological scoring of pancreatic tissue (edema, necrosis, inflammatory cell infiltration). (E) Serum amylase levels. (F) Serum lipase levels. CCK, cholecystokinin; AM, acetoxymethyl, represents living cells; PI, propidium iodide, represents dead cells. N ≥ 5, ns p ≥ 0.05, * p<0.05, **p<0.01, ***p<0.001