Fig. 5
From: Fibro-adipogenic progenitor cells in skeletal muscle unloading: metabolic and functional impairments
C2C12 myoblasts myogenic potential was changed under FAPs conditioned medium (a) Immunocytochemical staining of myotubes on the 5th day of exposure of the FAPs conditioned medium on C2C12 cells. Myotubes are visualized using MF20 antibody to myosin-heavy-chain (MyHC, green). Nuclei are stained with DAPI (blue). Scale bar 100 μm. (b) Fusion coefficient is calculated as a percent of nuclei incorporated in MF20 positive myotubes (n = 13 technical replicates, **** p < 0.0001). Data presented as mean ± SEM. (c) Width of C2C12 myotubes under FAPs medium (*** p < 0.001, **** p < 0.0001). Data presented as mean ± SEM. (d) A density plot of the number of nuclei per myotube. (e) Graphic representation of the contingency table of the number of nuclei in myotubes depending on the sample group: conditioned medium of Contr/HS7/HS14 FAPs