Fig. 2
From: Fibro-adipogenic progenitor cells in skeletal muscle unloading: metabolic and functional impairments
Functional unloading affects adipogenic differentiation of FAPs in vitro. (a) Representative images of lipid droplets OilRed O staining performed on the 11th day after adipogenic stimulation (red). Hematoxylin and eosin staining were used for nucleus and cytoplasm visualization (magnification 20x). (b) Image mask that converts red pixels in original image to yellow color. Purple color – background. (c) Quantitative analysis of intracellular lipids accumulation. Image analysis in Python was performed to estimate a number of red-stained pixels, which correlates with the total volume fat droplets in the samples (n = 40 technical replicates; ** p < 0.01, **** p < 0.0001). (d-g) qPCR analysis of key genes involved in adipogenic differentiation: Pparg, Fabp4, Atgl, Mgll. Expression analysis was performed on non-differentiated FAPs (d0) and on 3 and 7 days after induction of adipogenic differentiation (d3 and d7); n = 4 biological replicates; # show significant differences in HS7 vs. Contr and HS14 vs. Contr comparisons; * p < 0.05