Fig. 1
From: Fibro-adipogenic progenitor cells in skeletal muscle unloading: metabolic and functional impairments
Functional unloading affects FAP’s expansion and migration activity of FAPs purified from m. soleus and expanded in vitro. (a) Wound healing dynamic: wound area was measured at indicated time points and normalized to area in time point 1. Data represented as mean value (n = 6 biological replicates) and trend line shown in grey (95% confidence interval). (b) Wound area estimated at time points 10–20 and analyzed for HS and Control samples (n = 6 biological replicates, *p < 0.05). (c) Representative tracing images of selected FAPs migration curves. (d) Migration rate of FAPs estimated from tracking images of individual cell (n > 1000, * p < 0.05, **** p < 0.0001). (e) The dynamics of cellular growth of Contr/HS7/HS14 samples of FAPs between first and fourth passage. Data represented as mean ± SEM (n = 3 biological replicates, # show significant differences between HS7 and Contr samples, ## show significant differences in HS7 vs. Contr and HS14 vs. Contr comparisons. (f) FAPs population doubling times between second and fourth passages. Data represented as mean ± SEM (n = 3 biological replicates, # indicate significant differences between HS7 and Contr samples)