Fig. 4

Low level of DUX4 expression in myoblasts are unaffected by SIX1/2/4 knockdown. FSHD1 (54–2) were transfected with SIX1, SIX2, and SIX4 siRNAs in proliferating myoblasts 72 h before harvesting. qPCR results show relative mRNA expression for A and C DUX4, A-C DUX4 targets and D siRNAs targeting each respective SIX target. E-F Effects on SIX1 and SIX2 targets (PGK1, SLC4A7 and EYA1) were analyzed in E myoblasts and F myotubes. All conditions were carried out in triplicates with the mean and standard deviation depicted. Each experimental group (n = 3) was normalized to a negative si-Control with the mean and standard deviation depicted. Asterisks demonstrate statistical significance between the control vs experimental group using an unpaired two tailed t-test (ns = not significant; *p < 0.05)