Patient code | CYT VS NUCa | Ratiob |
|---|
101 − 011 | 1.3 vs. 0.9 | 1.4 CYT |
101 − 020 | 1.4 vs. 0.7 | 2 CYT |
101 − 017 | 1.1 vs. 0.5 | 2.2 CYT |
101-006 | 1.5 vs. 0.4 | 3.6 CYT |
101 − 018 | 0.9 vs. 1.2 | 0.8 NUC |
101-008 | 0.9 vs. 1.9 | 0.5 NUC |
101 − 014 | 0.4 vs. 2 | 0.2 NUC |
101 − 013 | 2 vs. 0.8 | 2.5 CYT |
- acolumn “CYT VS NUC” list the normalized number of BaseScope dots found in cytoplasm (first value) and in nuclei (second value), using respectively the following formula:\(\begin{array}{cc}\frac{\mathrm{Cytoplasmatic}\;\mathrm{dots}\;\mathrm{skipped}}{\mathrm{Total}\;\mathrm{dots}\;\mathrm{skipped}}/&\frac{\mathrm{Cytoplasmatic}\;\mathrm{dots}\;\mathrm{unskipped}}{\mathrm{Total}\;\mathrm{dots}\;\mathrm{unskipped}}=\mathrm{CYT}\\\frac{\mathrm{Nuclear}\;\mathrm{dots}\;\mathrm{skipped}}{\mathrm{Total}\;\mathrm{dots}\;\mathrm{skipped}}&/\frac{\;\;\;\;\;\;\;\;\;\;\;\mathrm{Nuclear}\;\mathrm{dots}\;\mathrm{unskipped}}{\mathrm{Total}\;\mathrm{dots}\;\mathrm{unskipped}}=\mathrm{NUC}\end{array}\)
- bcolumn “Ratio” lists the ratio between cytoplasmatic and nuclear transcripts and the preferential localization of the more expressed transcript.
