Fig. 2

Increased MyoD expression in proliferating HeyL-KO MuSCs. (A) Experimental scheme for analyzing the effect of HeyL-KO on MyoD expression in MuSCs on isolated myofibers from plantaris muscles 7 days after voluntary wheel running. (B) Number of wheel rotations during 7 days in WT (n = 5) or KO (n = 7) mice. (C and D) Immunostaining of Pax7 (red), MyoD (green), and Ki67 (white) in MuSCs on isolated myofibers at 7 days after wheel running from WT (C) or KO (D) mice. Nuclei were counterstained with DAPI. Scale bar, 100 μm. (E) Percentage of MyoD⁺ cells in Pax7⁺ cells (left) or Pax7⁺Ki67⁺ (right) cells on isolated myofibers from exercised WT (n = 4) or KO (n = 5) mice. (F) Number of Pax7⁺ cells on isolated myofibers from exercised WT (n = 4) or KO (n = 5) mice. (G) Percentage of Ki67⁺ cells in Pax7⁺ cells on isolated myofibers from exercised WT (n = 4) or KO (n = 5) mice