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Fig. 5 | Skeletal Muscle

Fig. 5

From: Isolation of small extracellular vesicles from regenerating muscle tissue using tangential flow filtration and size exclusion chromatography

Fig. 5

Purified muscle-derived EVs with Tangential Flow Filtration and SEC. AUpper panel. Quantification of EVs concentration on the different fractions collected upon SEC. Lower panel. EVs size distribution graphs for F3 to F7 upon SEC, showing how fraction F4 to F6 correlate with small EVs size (fraction of interest) and not before (F3) or after (F7). B Immunoblot analysis showing the expression enrichment of EVs markers on muscle-derived EVs. C Representative TEM pictures of isolated EVs derived from new regenerating hindlimb muscles using post-embedding techniques. Representative of 3 independent experiments. Size Exclusion Chromatography (SEC), Fraction (F), Transmission Electron Microscopy (TEM)

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Skeletal Muscle

ISSN: 2044-5040

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