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Fig. 2 | Skeletal Muscle

Fig. 2

From: Isolation of small extracellular vesicles from regenerating muscle tissue using tangential flow filtration and size exclusion chromatography

Fig. 2

Wnt7a is secreted on the surface of EVs upon a muscle injury. A Representative TEM image of a muscle transversal projection showing EVs secretion 96 h post injury. Uninjured muscle (1) containing quiescent satellite cells (2), does not secrete or uptake EVs. Conversely, new regenerating myofibers secrete EVs (3) presumably targeting and infiltrating distal injured muscle to exert a myoregenerative response (4). B Representative anti-Wnt7a iTEM labeling of a new regenerative myofiber transversal projection showing MVBs containing Wnt7a-expressing ILVs 96 h post injury. C Representative anti-Wnt7a iTEM labeling of an injured myofiber showing Wnt7a-expressing EVs infiltration 96 h post injury. Transmission Electron Microscopy (TEM), Extracellular Vesicles (EVs), Multi Vesicular Body (MVB), Intra Luminal Vesicles (ILV). D Workflow protocol of small EVs isolation derived from muscle injured tissue explants

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Skeletal Muscle

ISSN: 2044-5040

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