Fig. 4

Fiber size of the gastrocnemius muscle is reduced during chronic heart failure. Total cross-sectional area of the gastrocnemius muscle was analyzed by immunohistochemistry in C57BL/6J mice subjected to sham or transverse aortic constriction surgeries for 14 weeks. (A) Representative photomicrographs of transverse gastrocnemius muscle sections after anti-laminin staining; scale bar, 50 µm. (B) Total cross-sectional area normalized to total cell counts. Data are presented as the mean ± SEM; n = 5 samples in each group; *p = 0.04 vs sham