Table 1 Sequence and genomic location of the on-target and potential off-targets regions
From: Deletion of exons 45 to 55 in the DMD gene: from the therapeutic perspective to the in vitro model
Name | Locationa | Geneb | Sequence (5´-3´)c |
|---|---|---|---|
gRNA_44.1 On | ChrX: 32,038,608–32,038,631 (+) | DMD (ENSG00000198947) | AGCAAACAAGGTAACCCTGGAGG |
gRNA_44.1 Off1 | Chr2:105083351–105,083,374 (-) | MRPS9 (ENSG00000135972) | AGgAAAggAGGTAACCCTGGTGG |
gRNA_44.1 Off2 | Chr2: 14,281,851–14,281,874 (+) | LINC00276 (ENSG00000230448) | AtCcAACAAGaTAACCCTGGGGG |
gRNA_44.1 Off3 | Chr9: 21,835,997–21,836,020 (-) | MTAP (ENSG00000099810) | AGCAAAgAgGGTAgCCCTGGGGG |
gRNA_55.2 On | ChrX: 31,581,248–31,581,271 (-) | DMD (ENSG00000198947) | AGGAAAATTCGCAGACAGGCAGG |
gRNA_55.2 Off1 | Chr5: 157,057,959–157,057,982 (+) | HAVCR1 (ENSG00000113249) | AGGAAAATgaGCAGACAGGCTGG |
gRNA_55.2 Off2 | Chr11: 94,916,762–94,916,785 (+) | lncRNA (ENSG00000256469) | AGGAAAATTtaCAGACAGGaTGG |
gRNA_55.2 Off3 | Chr6: 111,393,626–111,393,649 (-) | REV3L (ENSG00000009413) | AGGAAgATaCGaAGACAGGCTGG |
gRNA_55.2 Off4 | Chr2: 238,379,513–238,379,536 (-) | TRAF3IP1 (ENSG00000204104) | AGGAAAATTCaCAtACAGGaTGG |