Fig. 6
From: Deletion of exons 45 to 55 in the DMD gene: from the therapeutic perspective to the in vitro model
Time course mRNA expression of myogenic markers during the differentiation process. The relative expression of Myf5 (A), MyoD (B) and Myh3 (C) was determined by ddPCR out at day d0, d2, d5 and d7 of differentiation in Control (C1), unedited DMD, the Edited∆45–55 and the Im∆45–55-D1 cell lines myotubes’ cDNA (n = 6 replicates). MyoD and Myf5 (FAM labelled) values were normalised to TBP (HEX labelled) while those for Myh3 (HEX labelled probe) to HPRT1 (FAM labelled). The error bars represent ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, according to the Kruskal-Wallis test at each differentiation time point