Fig. 5
From: Deletion of exons 45 to 55 in the DMD gene: from the therapeutic perspective to the in vitro model
Evaluation of the myogenic differentiation process after gene edition. Representative confocal images of Desmin (A) and MyHC (C) (myosin heavy chain) immunofluorescence (green) in control (C1), DMD, Edited∆45–55 and Im∆45–55-D1 at differentiation days 0, 2, 5 and 7(d0, d2, d5, d7). D0 images of MyHC staining are not shown (negatively stained). Nuclei were stained with DAPI (blue) (scale bar = 50 μm). (B) Quantification of the myogenic fusion index and myotube diameter of the cell lines was calculated using the Desmin micrographs (10 images). (D) Quantification of the percentage of nuclei within MyHC + cells in each condition (10 images, 300–500 total nuclei). The bar graphs show the mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, according to the Kruskal-Wallis test at each differentiation time point