Fig. 3
From: Deletion of exons 45 to 55 in the DMD gene: from the therapeutic perspective to the in vitro model
Dystrophin cDNA expression analysis of the edited clone. (A) Confirmation of the del45–55-D1 of the differentiated myotubes cDNA from (1) the Im∆45–55-D1 and (2) from the Edited∆45–55 clone. In both samples the exon 56 sequence is juxtaposed to the exon 44 one. (B) Dystrophin expression quantification through ddPCR of the differentiated myotubes cDNA from Control (C1), unedited DMD, the Edited∆45–55 and the Im∆45–55-D1 cell lines (n = 8 replicates). The bar graphs represent mean ± SEM. *p < 0.05, ***p < 0.001, according to Kruskal-Wallis test