Fig. 8
From: Muscle degeneration in aging Drosophila flies: the role of mechanical stress
Fiber degeneration persists in non-excitable TDT muscles. A, B TDT organization in young flies was revealed by F-actin (green), Integrin (red) and nuclear (blue) staining. No changes in muscle cross-sectional area and fiber layout are evident between experimental (Ca-α1D KD) and control (CNTR) flies. B A closeup of several TDT fibers from young flies stained for F-actin (green), T-tubules (Dlg, red), and nuclei (blue). Organization of myonuclei and myofibrils is perturbed in live TDT fibers of Ca-α1D KD flies. Arrowheads indicate nucleus-containing lacunae in the control muscle. C Jump test results. Ca-α1D KD flies completely lose the ability to jump. Statistical significance by Student’s t-test: (***) p < 0.001. D Degeneration profiles of the TDT muscles in old 4 wo flies. No statistically significant differences between control (CNTR) and experimental (Ca-α1D KD) groups were found by Fisher’s exact test. Scale bars: 50 µm (A), 20 µm (B)