Fig. 5

Quantification of spontaneous fiber degeneration in the TDT muscle. A Survivorship of w¹¹¹⁸ flies reared at 29 °C. Under these conditions, the maximal lifespan is 6 weeks. B Box plots of live fiber counts in the TDTs of young (0 week old, wo) and old (5 wo) flies of the w¹¹¹⁸ (dark grey) and y w (light grey) genetic lines. Crosses depict position of the mean, whiskers are determined by Tukey test, notches show the 95% confidence interval. C Data that was re-drawn from B as the average loss of live TDT fibers in old flies. Whiskers show standard deviation. D Examples of fiber degeneration in the TDT muscle (outlined by dashed line) with assigned degeneration scores. Degenrative fibers are marked by X.  E Quantification of TDT degeneration based on degeneration scores. The area of each circle represents the fraction of muscles received a particular penalty score. Statistical significance by Fisher’s Exact Test: (*) p < 0.05; (***) p < 0.001. Scale bars: 50 µm