Fig. 3
From: Muscle degeneration in aging Drosophila flies: the role of mechanical stress
Degenerative fibers in Drosophila muscles show signs of necrosis. A-C Accumulation of injected vital dye trypan blue in degenerative fibers of IFM (A), subalar (B), and TDT (C) muscles. D-D’: A degenerative TDT fiber that lacks F-actin and DNA staining (D) does not show enhanced accumulation of apoptosis-selective dye toluidine blue in a serial section (D’). “X” marks degenerative fibers as determined by reduced or lacking staining for F-actin (green) and DNA (blue). E Mitochondrial swelling in two degenerative TDT muscle fibers (outlined) expressing mito-GFP (red). Scale bars: 50 µm (all panels)