Fig. 6
Relative immune-related gene expression in quadriceps and psoas muscles of wild-type (WT) C57Bl/6J and dysferlin-deficient BLA/J mice aged 10 months, without and with dexamethasone (Dex) treatment. A, H Tumour necrosis factor (Tnf); B, I Complement C1q B Chain (C1qb); C, J Complement component 3 (C3); D, K Complement component 4 (C4); E, L Complement C5a Receptor 1 (C5ar1); F, M Caspase 1 (Casp1); G, N NLR family pyrin domain containing 3 (Nlrp3). All mRNA expression values were standardised against the reference genes peptidylprolyl isomerase A (Ppia) and TATA-box binding protein (Tbp1) using the GeNorm algorithm [64]. Data were analysed by two-way ANOVA: *, **, **** BLA/J (± Dex) vs WT (± Dex) (p < 0.05, 0.01, 0.0001, respectively, strain effect); # Dex-treated vs untreated (p < 0.05, treatment effect); ^, ^^^, ^^^^ significant difference between groups (p < 0.05, 0.001, 0.0001, respectively, strain/treatment interaction effect). Data are log10 transformed and presented on a log10 y-axis scale as mean ± SD (n = 4–10)