Fig. 2
HMOX1 expression in induced by TX in SCs of mdx;HMOX1Pax7Ind mice. Quantitative analysis of the percentage of HO-1 positive cells among (A) CD45 + CD31- (WBCs), (B) CD45-CD31-Sca-1 + cells, and (C) representative plots and (D) quantitative analysis of the percentage of HO-1 positive cells among SCs in mdx and mdx;HMOX1Pax7Ind hind limb skeletal muscles. Flow cytometry (n = 5–8). Data is presented as mean± SEM. ## p < 0.01, ### p < 0.001—Two-way ANOVA interaction; *** p < 0.001 by Two-way ANOVA with Tukey’s post hoc test. (F) HO-1 is expressed and colocalizes with Pax7 expression in TX-treated mdx;HMOX1Pax7Ind but not in mdx mice. Immunofluorescence of Pax7 (green), HO-1 (red) and nuclei (DAPI, blue). Scale bar: 5 μm