Fig. 7
Mbnl1ΔE3/ΔE3 muscle shows resistance to atrophy upon denervation. A Mass variation after 3 weeks of denervation in control and Mbnl1ΔE3/ΔE3 mice, for TA and gastrocnemius (GA) muscles. n = 4 per group. B Fluorescent images of denervated muscles from control and Mbnl1ΔE3/ΔE3 mice, stained with antibodies against type IIA myosin heavy chain (MHC2A - red) and laminin (green) and quantification of fibre type proportion in control and mutant denervated muscles. Scale bar, 500 μm. n = 4 mice per group. C Quantification of fibre minimum ferret in innervated and 3-week-denervated muscles from control and Mbnl1ΔE3/ΔE3 mice. n = 4 mice per group. D Fibre size distribution for type IIA, IIX and IIB fibres in innervated and 3-week-denervated muscles from Mbnl1ΔE3/ΔE3 and control mice. n = 4/4 Ctrl/Mbnl1ΔE3/ΔE3. All data are mean ± SEM; $p < 0.05, $$p < 0.01, $$$p < 0.001, $$$$p < 0.0001 between Inn/Den; * p < 0.05, ** p < 0.01, **** p < 0.0001 between genotypes; two-way ANOVA with a Tukey’s post-hoc analysis (Inn/Den and Ctrl/ Mbnl1ΔE3/ΔE3; C) or two-tailed unpaired Student’s t-test (A, B)