Fig. 6
Changes in HDAC4 signalling pathway in Mbnl1ΔE3/ΔE3 and HSALR muscles. A, B Western blot analysis of HDAC4 and its phosphorylated form (Ser632) in total protein lysate of Mbnl1ΔE3/ΔE3 and HSALR muscles. Quantification of total levels is given in B. Quantification of phosphorylated levels is given in Supplementary Material, Fig. S8B. Total levels are normalized to α-actinin. n = 3 per group. C, D Western blot analysis of HDAC4 in nuclear (C, D) and cytosolic (E, F) fractions of gastrocnemius muscle from Mbnl1ΔE3/ΔE3 mice and HSALR mice. Quantifications of HDAC4 levels are given in D and F. Levels are normalized to lamin-B (D) and tubulin (F). n = 5/4 Ctrl/Mbnl1ΔE3/ΔE3 and 3/4 Ctrl/HSALR. G mRNA levels of Dach2, Actc1, Ramp1, Dhrs7c, Cdh1 and Mitr in TA muscle from Mbnl1ΔE3/ΔE3 mice and in gastrocnemius muscle from HSALR mice. Transcript levels are normalized to Tbp. n = 4/3 Ctrl/Mbnl1ΔE3/ΔE3 (except for Dhrs7c and Cdh1, n = 6/5) and 4/4 (Dach2/Mitr), 6/6 (Actc1/Cdh1), 6/5 (Ramp1/Dhrs7c) Ctrl/HSALR. All protein (B, D, F) and RNA (G) levels are relative to control and expressed as log2(Fold Change). All data are mean ± SEM; * p < 0.05; ** p < 0.01; ****p < 0.0001; two-tailed unpaired Student’s t-test